Composite

Part:BBa_K4662071:Design

Designed by: Julia Wyss   Group: iGEM23_UZurich   (2023-10-12)


sgRNA to block Pyruvate formate-lyase 1-activating enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Check if part is compatible with assemlby standards RFC 1000.


Source

This composite parts contains an constitutive anderson promoter BBa_J23119. The crRNA was designed and its efficiency was predicted (68%) with the webpage chopchop and contains the PAM sequence in the promoter region of the Pyruvate formate-lyase 1-activating. The tracrRNA was implemented from S. pyogenes BBa_K4662031


References