Composite
Part:BBa_K4662071:Design
Designed by: Julia Wyss Group: iGEM23_UZurich (2023-10-12)
sgRNA to block Pyruvate formate-lyase 1-activating enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Check if part is compatible with assemlby standards RFC 1000.
Source
This composite parts contains an constitutive anderson promoter BBa_J23119. The crRNA was designed and its efficiency was predicted (68%) with the webpage chopchop and contains the PAM sequence in the promoter region of the Pyruvate formate-lyase 1-activating. The tracrRNA was implemented from S. pyogenes BBa_K4662031